Transcript profiling of sucrose synthase genes involved in sucrose metabolism among four carrot (Daucus carota L.) cultivars reveals distinct patterns
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منابع مشابه
Molecular characterization and functional analysis of sucrose-cleaving enzymes in carrot (Daucus carota L.).
The amount of carbon transported into storage organs of crop plants to a large degree determines crop yield. The role of sucrose-cleaving enzymes in this process is not clear and it is the main goal of our work to tackle this question. Sucrose cleavage is catalysed either by invertase or sucrose synthase both of which exist in several isoforms with different subcellular locations. Carrot (Daucu...
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The metabolism of exogenously added d-myo-[1-(3)H]inositol 1,4,5-trisphosphate (IP(3)) has been examined in microsomal membrane and soluble fractions of carrot (Daucus carota L.) cells grown in suspension culture. When [(3)H]IP(3) was added to a microsomal membrane fraction, [(3)H]IP(2) was the primary metabolite consisting of approximately 83% of the total recovered [(3)H] by paper electrophor...
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We have shown previously that inositol-1,4,5-trisphosphate (IP(3)) stimulates an efflux of (45)Ca(2+) from fusogenic carrot protoplasts (M Rincón, WF Boss [1987] Plant Physiol 83: 395-398). In light of these results, we suggested that IP(3) might serve as a second messenger for the mobilization of intracellular Ca(2+) in higher plant cells. To determine whether or not IP(3) and other inositol p...
متن کاملBiochemical, physiological, and molecular characterization of sucrose synthase from Daucus carota.
Sucrose synthase (EC 2.4.1.13) from carrot (Daucus carota) is a tetramer with a molecular mass of 320 kD and subunits of 80 kD. The enzyme has a pH optimum of 7.0 (cleavage direction). Maximal activities were measured at 55 degrees C. The Km for Suc was estimated as 87 mM and for UDP as 0.39 mM. Fructose acts as a noncompetitive inhibitor with an inhibition constant of 17.2 mM. In contrast, glu...
متن کاملGuaiacol Peroxidases in Carrot (Daucus carota L.) Root**
Carrot (Daucus carota L.) belongs to the group of common edible vegetables. The aim of the present study was to establish the distribution of guaiacol peroxidases in carrot root sections as well as to determine the enzymatic activity and the electrophoretic pattern of isoperoxidases in tissue extracts of particular root regions: central cylinder, inner cortex and epidermis, including the periph...
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ژورنال
عنوان ژورنال: BMC Plant Biology
سال: 2018
ISSN: 1471-2229
DOI: 10.1186/s12870-017-1221-1